Application

The antibody is suitable for use on both frozen and protease digested paraffin processed tissue.

SUGGESTED USAGE

Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:40 will be applicable to most commonly used systems.

Optimal working dilutions must be determined by the end user.

Detect Collagen Type IV using this Anti-Collagen Type IV Antibody, clone 24.12.8 (PHM-12) validated for use in IH, IH(P).

General description

BIOCHEMISTRY:

p I = 5.5

Immunogen

Human glomeruli

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified immunoglobulin presented as a liquid in phosphate buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide.

Format: Purified

Specificity

MAB1430 provides a marker for an antigen known to be important in glomerular cell-matrix interactions, and should prove useful in research into the mechanisms involved in renal pathology. This antibody reacts with glomerular and tubular basement membranes in kidney as well as basal lamina of capillaries. It also labels mesangial cells and matrix within the glomerulus as well as basement membrane structures in all organs examined (Gusterson et al., 1984).

Storage and Stability

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.